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Wound Pathogens Assay

A high-performance multiplex real-time qPCR assay designed for the simultaneous detection of wound-associated bacterial, fungal, anaerobic, and atypical pathogens, along with select antimicrobial resistance markers. The Assay is provided in an all-in-one tube format containing pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex amplification per reaction, with RNase P included as an internal control. The assay is engineered for low-copy target detection, high amplification efficiency, and minimal cross-reactivity in complex polymicrobial samples. Compatible with standard real-time PCR platforms and fluorescent detection channels, the panel is intended for use with purified nucleic acids extracted from a wide range of wound and soft-tissue specimen types in research and assay development settings.

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Assays
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Targets

Targets:

  • Escherichia coli

  • Staphylococcus aureus

  • Staphylococcus epidermidis

  • Klebsiella pneumoniae

  • Klebsiella oxytoca

  • Klebsiella aerogenes

  • Enterobacter cloacae

  • Citrobacter freundii

  • Serratia marcescens

  • Proteus mirabilis

  • Proteus vulgaris

  • Morganella morganii

  • Pseudomonas aeruginosa

  • Acinetobacter baumannii

  • Group A Streptococcus

  • Group B Streptococcus

  • Group C & G Streptococcus

  • Enterococcus faecalis

  • Enterococcus faecium

  • RNase P (internal control)

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Targets:

  • Escherichia coli

  • Staphylococcus aureus

  • Staphylococcus epidermidis

  • Klebsiella pneumoniae

  • Klebsiella oxytoca

  • Klebsiella aerogenes

  • Enterobacter cloacae

  • Citrobacter freundii

  • Serratia marcescens

  • Proteus mirabilis

  • Proteus vulgaris

  • Morganella morganii

  • Pseudomonas aeruginosa

  • Acinetobacter baumannii

  • Group A Streptococcus

  • Group B Streptococcus

  • Group C & G Streptococcus

  • Enterococcus faecalis

  • Enterococcus faecium

  • RNase P (internal control)

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Targets:

  • Bacteroides fragilis

  • Prevotella nigrescens

  • Prevotella intermedia

  • Peptostreptococcus anaerobius

  • Anaerococcus prevotii

  • Cutibacterium acnes

  • Finegoldia magna

  • Peptoniphilus asaccharolyticus

  • Bartonella henselae

  • RNase P (internal control)

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Targets:

  • Candida albicans

  • Candida tropicalis

  • Candida parapsilosis

  • Candida dubliniensis

  • Candida glabrata

  • Candida auris

  • Candida krusei

  • Candida lusitaniae

  • Mycobacterium fortuitum

  • Mycobacterium chelonae

  • Vibrio vulnificus

  • RNase P (internal control)

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Targets:

  • Gram-Positive / Virulence

    • mecA

    • vanA1 / vanA2

    • vanB

    • PVL

    • gyrA

  • Carbapenemases

    • KPC

    • NDM

    • VIM

    • IMP-7

    • OXA-48

    • OXA-23

    • OXA-24/40

  • ESBL / Quinolone Resistance

    • CTX-M Group 1

    • CTX-M Group 2

    • SHV

    • TEM

    • qnrA

    • qnrB

  • Macrolide / Tetracycline Resistance

    • tetS

    • tetM

    • ermA

    • ermB

    • ermC

  • RNase P (internal control)

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Provide us with the names of your targets, and Smyrna Bioscience will design and configure a customized multiplex qPCR panel to meet your research requirements. Panels are delivered in an all-in-one tube format with pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex detection per reaction. Assays are engineered for high analytical sensitivity, specificity, and compatibility across major real-time PCR platforms.

Nail Pathogens Assay

A high-performance multiplex real-time qPCR assay designed for the simultaneous detection of clinically relevant dermatophytes, yeasts, non-dermatophyte molds, and select bacterial pathogens associated with nail and periungual infections, with optional antimicrobial resistance markers. The assay is provided in an all-in-one tube format containing pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex amplification per reaction, with RNase P included as an internal control. The assay is engineered for sensitive low-copy target detection, high amplification efficiency, and minimal cross-reactivity in keratin-rich and polymicrobial nail specimens. Compatible with standard real-time PCR platforms and common fluorescent detection channels, the panel is intended for use with purified nucleic acids extracted from nail clippings, subungual debris, and related specimen types in research and assay development settings.

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Assays
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Targets

Targets:

  • Trichophyton spp

  • Trichophyton interdigitale

  • Trichophyton tonsurans

  • Epidermophyton floccosum

  • Microsporum audouinii / Microsporum canis

  • Microsporum gypseum

  • Microsporum nanum

  • RNase P (Internal Control)

Targets:

  • Candida albicans

  • Candida glabrata

  • Candida tropicalis

  • Candida parapsilosis

  • Candida krusei

  • Candida dubliniensis

  • Candida lusitaniae

  • Candida auris

  • Malassezia furfur

  • Pichia onychis

  • RNase P

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Targets:

  • Scopulariopsis brevicaulis

  • Fusarium solani

  • Aspergillus niger

  • Aspergillus terreus

  • Acremonium strictum

  • Alternaria alternata

  • Curvularia lunata

  • Neoscytalidium dimidiatum

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Targets:

  • Staphylococcus aureus

  • Enterococcus faecalis

  • Escherichia coli

  • Klebsiella pneumoniae

  • Klebsiella oxytoca

  • Enterobacter cloacae

  • Pseudomonas aeruginosa

  • Citrobacter koseri

  • Group A Strep

  • Group B Strep

  • Group C & G Strep

  • Peptoniphilus / Peptostreptococcus asaccharolyticus

  • Mycobacterium fortuitum

  • RNase P

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Targets:

  • mecA

  • vanA1 / vanA2

  • vanB

  • ermA / ermC 

  • ermB

  • qnrA

  • qnrB

  • blaCMY-2

  • OXA-23 / OXA-24

  • tetM / tetS

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Provide us with the names of your targets, and Smyrna Bioscience will design and configure a customized multiplex qPCR panel to meet your research requirements. Panels are delivered in an all-in-one tube format with pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex detection per reaction. Assays are engineered for high analytical sensitivity, specificity, and compatibility across major real-time PCR platforms.

Respiratory Pathogens Assay

A high-performance multiplex real-time qPCR assay designed for the simultaneous detection of clinically relevant viral, bacterial, and atypical bacterial pathogens associated with upper and lower respiratory tract syndromes, with optional antimicrobial resistance markers. The assay is provided in an all-in-one tube format containing pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex amplification per reaction, with RNase P included as an internal control. The assay is engineered for sensitive low-copy target detection, high amplification efficiency, and minimal cross-reactivity in complex, polymicrobial respiratory specimens. Compatible with standard real-time PCR platforms and common fluorescent detection channels, the assay is intended for use with purified nucleic acids extracted from nasopharyngeal swabs, oropharyngeal swabs, sputum, and related respiratory specimen types in research and assay development settings.

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Assays
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Targets

Viruses

  • Adenovirus

  • Coronaviruses (229E, HKU1, NL63, OC43)

  • SARS-CoV-2 (COVID-19 Coronavirus)

  • Enterovirus D68

  • Human metapneumovirus

  • Influenza virus A

  • Influenza virus B

  • Parainfluenza virus (Types 1, 2, 3, 4)

  • Respiratory syncytial virus (RSV)

  • Rhinovirus / Enterovirus

Viruses

  • Adenovirus

  • Coronaviruses (229E, HKU1, NL63, OC43)

  • SARS-CoV-2 (COVID-19 Coronavirus)

  • Enterovirus D68

  • Human metapneumovirus

  • Influenza virus A

  • Influenza virus B

  • Parainfluenza virus (Types 1, 2, 3, 4)

  • Respiratory syncytial virus (RSV)

  • Rhinovirus / Enterovirus

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

  • SARS-CoV-2 (COVID-19 Coronavirus)

  • Influenza virus A

  • Influenza virus B

  • Respiratory syncytial virus (RSV)

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Bacterial

  • Streptococcus pyogenes (Group A Streptococcus)

  • Streptococcus dysgalactiae (Group C & G Streptococcus)

Atypical Bacteria

  • Chlamydia pneumoniae

  • Mycoplasma pneumoniae

Viral

  • Adenovirus

  • Coronaviruses (229E, HKU1, NL63, OC43)

  • SARS-CoV-2

  • Enterovirus D68

  • Human metapneumovirus

  • Influenza virus A

  • Influenza virus B

  • Parainfluenza virus (Types 1, 2, 3, 4)

  • Respiratory syncytial virus

  • Rhinovirus / Enterovirus

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Bacterial

  • Haemophilus influenzae

  • Moraxella catarrhalis

  • Streptococcus pneumoniae

Atypical Bacteria

  • Bordetella pertussis

  • Bordetella parapertussis

  • Bordetella bronchiseptica

  • Chlamydia pneumoniae

  • Mycoplasma pneumoniae

Viral

  • Adenovirus

  • Coronaviruses (229E, HKU1, NL63, OC43)

  • SARS-CoV-2

  • Enterovirus D68

  • Human metapneumovirus

  • Influenza virus A

  • Influenza virus B

  • Parainfluenza virus (Types 1, 2, 3, 4)

  • Respiratory syncytial virus

  • Rhinovirus / Enterovirus

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

  • Trimethoprim / Sulfonamide Resistance

    • dfr (A1, A5)

    • sul (1, 2)

  • Macrolide Resistance

    • ermB

    • ermC

    • mefA

  • Tetracycline Resistance

    • tetB

    • tetM

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Provide us with the names of your targets, and Smyrna Bioscience will design and configure a customized multiplex qPCR panel to meet your research requirements. Panels are delivered in an all-in-one tube format with pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex detection per reaction. Assays are engineered for high analytical sensitivity, specificity, and compatibility across major real-time PCR platforms.

Gastrointestinal Pathogens Assay

A high-performance multiplex real-time qPCR assay designed for the simultaneous detection of clinically relevant bacterial and viral pathogens associated with acute gastrointestinal illness, with optional antimicrobial resistance markers. The assay is provided in an all-in-one tube format containing pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex amplification per reaction, with RNase P included as an internal control. The assay is engineered for sensitive low-copy target detection, high amplification efficiency, and minimal cross-reactivity in complex, inhibitor-rich stool matrices. Compatible with standard real-time PCR platforms and common fluorescent detection channels, the assay is intended for use with purified nucleic acids extracted from stool and related gastrointestinal specimen types in research and assay development settings.

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Assays
0 +
Targets

Viruses

  • Adenovirus

  • Coronaviruses (229E, HKU1, NL63, OC43)

  • SARS-CoV-2 (COVID-19 Coronavirus)

  • Enterovirus D68

  • Human metapneumovirus

  • Influenza virus A

  • Influenza virus B

  • Parainfluenza virus (Types 1, 2, 3, 4)

  • Respiratory syncytial virus (RSV)

  • Rhinovirus / Enterovirus

  • Campylobacter (coli, jejuni, upsaliensis)

  • Shigella spp. / Enteroinvasive E. coli (EIEC)

  • Enteropathogenic E. coli (EPEC)

  • Enterotoxigenic E. coli (ETEC)

  • Shiga toxin–producing E. coli (STEC)

  • STEC O157

  • Salmonella spp.

  • Vibrio (cholerae, parahaemolyticus, vulnificus)

  • Yersinia enterocolitica

  • Clostridioides difficile (toxin A/B)

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

  • Adenovirus F40/41

  • Norovirus (Genogroups I & II)

  • Rotavirus A 

  • Sapovirus 

  • Astrovirus 

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

  • Giardia lamblia

  • Cryptosporidium spp.

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

β-lactam / ESBL / AmpC

  • ACT, MIR, FOX, ACC groups

  • CTX-M1 (15), M2 (2), M9 (9), M8/25 groups

  • SHV group

Carbapenemases

  • KPC group

  • IMP group

  • NDM group

  • VIM group

  • OXA-48

  • OXA-51

Other Resistance

  • qnrA1, qnrA2, qnrB2

  • ermB, ermC, mefA

  • dfrA1, dfrA5

  • sul1, sul2

  • tetB, tetM

*Up to five targets per vial (including RNase P). Each vial represents an independent multiplex qPCR assay.

Provide us with the names of your targets, and Smyrna Bioscience will design and configure a customized multiplex qPCR panel to meet your research requirements. Panels are delivered in an all-in-one tube format with pre-optimized primers, probes, and reaction chemistry, supporting up to 5-plex detection per reaction. Assays are engineered for high analytical sensitivity, specificity, and compatibility across major real-time PCR platforms.

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